Part:BBa_K1429003:Experience

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Applications of BBa_K1429003

We have put this biobrick under the control of the T7 promoter by cloning the biobrick part into pUC19. The expression of the OFPYukon can be seen in the photograph. It is clone OFP2:

The lysis buffer from the Carolina Biological kit "Purification of Green Fluorescent Protein" was used to lyse cells from an overnight 2mL culture of E. coli K-12 Top10 cells containing pUC19 with this biobrick. The lysate was analyzed by fluorimetry to determine if the excitation and emission wavelengths matched the reported ones of 550 and 563 respectively. They did, confirming the protein was the correct one.

User Reviews

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